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phix illumina spike  (Illumina Inc)


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    Structured Review

    Illumina Inc phix illumina spike
    Phix Illumina Spike, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 3881 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phix+spike/PhiX+Control+v3/med_rxiv__64898__2026__10__13__26364774-235-10-11
    Average 99 stars, based on 3881 article reviews
    phix illumina spike - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    Concentration Assay:

    Article Title: Efficacies of three anti-Salmonella additives - bacteriophage, formaldehyde, and formic acid - within the animal food microbiota
    Article Snippet: Briefly, the PCR reaction was carried out using the 1× KAPA HiFi HotStart ReadyMix (Roche, Indianapolis, IN, USA) and the libraries were prepared using the Nextera XT Index Kit (Illumina, San Diego, CA, USA). .. The normalized libraries (4 nM each) were pooled, denatured, and loaded onto the MiSeq (Illumina) at a final pooled library concentration of 4 pM, and sequenced with 40% PhiX spike-in using the MiSeq Reagent Kit v3 (600 cycles; Illumina). ..

    Article Title: Staphylococcus haemolyticus Population Genomics Provides Insights into Pathogenicity and Commensalism
    Article Snippet: DNA was quantified using the Quant-iT TM dsDNA HS assay (ThermoFisher), the fluorescence was measured on a FLUOstar Optima plate reader set at 480/530 nm (excitation/emission) .. The pool was run at a final concentration of 1.5 pM on an Illumina Nextseq500 instrument using a Mid Output Flowcell (NSQ® 500 Mid Output KT v2(300 CYS) Illumina Catalogue FC-404-2003) following the Illumina recommended denaturation and loading recommendations which included a 1% PhiX spike in (PhiX Control v3 Illumina Catalogue FC-110-3001). .. The pool was run at a final concentration of 1.5 pM on an Illumina Nextseq500 instrument using a Mid Output Flowcell (NSQ 500 Mid Output KT v2(300 CYS) Illumina Catalogue FC-404-2003) following the Illumina recommended denaturation and loading recommendations which included a 1% PhiX spike in (PhiX Control v3 Illumina Catalogue FC-110-3001).

    Article Title: SAS eDNA complements traditional sampling in detecting fish diversity along river-estuarine-coastal continuum
    Article Snippet: Traditional morphological species identification (MSI) in estuarine ecosystems remains limited by sampling and detection bias, particularly for fish along river to marine ecosystems.. We compared sequence assignment species environmental DNA (SAS eDNA) metabarcoding and MSI for fish assemblage characterization across the Seomjin River estuary and Gwangyang Bay in South Korea.. We conducted the study at six sites in May and September 2022 using gill nets and beam trawling for MSI, water samples were collected for SAS eDNA analysis using 12S rRNA primers.

    Article Title: Hologenomic analysis of rectal mucus sampling for detection of adenomatous polyps and colorectal cancer.
    Article Snippet: .. The pool was run at a final concentration of 12 pMon an IlluminaMiSeq instrument usingMiSeq® Reagent Kit v3 (600 cycle) (Illumina Catalogue FC-102-3003) following the Illumina recommendeddenaturation and loading recommendationswhich included a 20% PhiX spike in (PhiX Control v3 Illumina Catalogue FC-110-3001). ..

    Article Title: Optimizing storage, high-molecular weight DNA extraction and genome reconstructions from human faecal samples
    Article Snippet: A single 0.7X SPRI clean-up using sample purification beads (Illumina® DNA Prep, (M) Tagmentation (96 Samples, IPB)) was performed on the pool. .. After the final quality check using Qubit and Tapestation, the pool was run at a final concentration of 750pM on an Illumina Nextseq 2000 instrument using a NextSeq 2000 P1 XLEAP-SBS Reagent Kit (600 Cycles) including a 20% PhiX spike in (PhiX Control v3 Illumina). .. Four additional stool samples from The Mucosodom human study participants, under approved ethics (REC: BAC.004.A2, 2020/21-006) were preserved with (S) and DNA extracted with Protocol 3 i.e., bead-beating via Lysis Matrix E (MP Biomedicals) and Vortex adapter for 24x2ml tubes for Vortex-GenieTM2 (10 min, highest speed) (Scientific Industries SITM) and the Maxwell PureFood GMO and Authentication Kit (Promega, AS1600), and sequenced using three technologies: Genomic DNA was normalised to 5ng/μl with EB (10mM Tris-HCl).

    Article Title: Hologenomic analysis of rectal mucus sampling for detection of adenomatous polyps and colorectal cancer
    Article Snippet: A final Qubit and sizing on a D5000 Screen Tape (Agilent Catalogue No. 5067-5579) using the Agilent Tapestation 4200 was done to calculate the final library pool molarity. .. The pool was run at a final concentration of 12 pM on an Illumina MiSeq instrument using MiSeq® Reagent Kit v3 (600 cycle) (Illumina Catalogue FC-102-3003) following the Illumina recommended denaturation and loading recommendations which included a 20% PhiX spike in (PhiX Control v3 Illumina Catalogue FC-110-3001). .. Nextera Flex Enzyme kit (Illumina, San Diego, USA) tagmentation master mix was prepared using 0.5 μl Bead-Linked Transposomes (BLT), 0.5 μl Tagmentation Buffer 1 (TB1) and 4 μl H20 for a total volume of 5 μl per reaction.

    Article Title: Gut microbial diversity impacts carbohydrate fermentation by children with severe acute malnutrition
    Article Snippet: A final Qubit and sizing on High Sensitivity D1000 Screen Tape (Agilent Catalogue No. 5067-5579) using the Agilent Tapestation 4200 was done to calculate the final library pool molarity. .. The pool was run at a final concentration of 10 pM on an Illumina MiSeq instrument using MiSeq® Reagent Kit v3 (600 cycle) (Illumina Catalogue FC-102-3003) following the Illumina recommended denaturation and loading recommendations, which included a 20% PhiX spike in (PhiX Control v3 Illumina Catalogue FC-110-3001). ..

    Control:

    Article Title: Staphylococcus haemolyticus Population Genomics Provides Insights into Pathogenicity and Commensalism
    Article Snippet: DNA was quantified using the Quant-iT TM dsDNA HS assay (ThermoFisher), the fluorescence was measured on a FLUOstar Optima plate reader set at 480/530 nm (excitation/emission) .. The pool was run at a final concentration of 1.5 pM on an Illumina Nextseq500 instrument using a Mid Output Flowcell (NSQ® 500 Mid Output KT v2(300 CYS) Illumina Catalogue FC-404-2003) following the Illumina recommended denaturation and loading recommendations which included a 1% PhiX spike in (PhiX Control v3 Illumina Catalogue FC-110-3001). .. The pool was run at a final concentration of 1.5 pM on an Illumina Nextseq500 instrument using a Mid Output Flowcell (NSQ 500 Mid Output KT v2(300 CYS) Illumina Catalogue FC-404-2003) following the Illumina recommended denaturation and loading recommendations which included a 1% PhiX spike in (PhiX Control v3 Illumina Catalogue FC-110-3001).

    Article Title: Hologenomic analysis of rectal mucus sampling for detection of adenomatous polyps and colorectal cancer.
    Article Snippet: .. The pool was run at a final concentration of 12 pMon an IlluminaMiSeq instrument usingMiSeq® Reagent Kit v3 (600 cycle) (Illumina Catalogue FC-102-3003) following the Illumina recommendeddenaturation and loading recommendationswhich included a 20% PhiX spike in (PhiX Control v3 Illumina Catalogue FC-110-3001). ..

    Article Title: Optimizing storage, high-molecular weight DNA extraction and genome reconstructions from human faecal samples
    Article Snippet: A single 0.7X SPRI clean-up using sample purification beads (Illumina® DNA Prep, (M) Tagmentation (96 Samples, IPB)) was performed on the pool. .. After the final quality check using Qubit and Tapestation, the pool was run at a final concentration of 750pM on an Illumina Nextseq 2000 instrument using a NextSeq 2000 P1 XLEAP-SBS Reagent Kit (600 Cycles) including a 20% PhiX spike in (PhiX Control v3 Illumina). .. Four additional stool samples from The Mucosodom human study participants, under approved ethics (REC: BAC.004.A2, 2020/21-006) were preserved with (S) and DNA extracted with Protocol 3 i.e., bead-beating via Lysis Matrix E (MP Biomedicals) and Vortex adapter for 24x2ml tubes for Vortex-GenieTM2 (10 min, highest speed) (Scientific Industries SITM) and the Maxwell PureFood GMO and Authentication Kit (Promega, AS1600), and sequenced using three technologies: Genomic DNA was normalised to 5ng/μl with EB (10mM Tris-HCl).

    Article Title: Hologenomic analysis of rectal mucus sampling for detection of adenomatous polyps and colorectal cancer
    Article Snippet: A final Qubit and sizing on a D5000 Screen Tape (Agilent Catalogue No. 5067-5579) using the Agilent Tapestation 4200 was done to calculate the final library pool molarity. .. The pool was run at a final concentration of 12 pM on an Illumina MiSeq instrument using MiSeq® Reagent Kit v3 (600 cycle) (Illumina Catalogue FC-102-3003) following the Illumina recommended denaturation and loading recommendations which included a 20% PhiX spike in (PhiX Control v3 Illumina Catalogue FC-110-3001). .. Nextera Flex Enzyme kit (Illumina, San Diego, USA) tagmentation master mix was prepared using 0.5 μl Bead-Linked Transposomes (BLT), 0.5 μl Tagmentation Buffer 1 (TB1) and 4 μl H20 for a total volume of 5 μl per reaction.

    Article Title: Gut microbial diversity impacts carbohydrate fermentation by children with severe acute malnutrition
    Article Snippet: A final Qubit and sizing on High Sensitivity D1000 Screen Tape (Agilent Catalogue No. 5067-5579) using the Agilent Tapestation 4200 was done to calculate the final library pool molarity. .. The pool was run at a final concentration of 10 pM on an Illumina MiSeq instrument using MiSeq® Reagent Kit v3 (600 cycle) (Illumina Catalogue FC-102-3003) following the Illumina recommended denaturation and loading recommendations, which included a 20% PhiX spike in (PhiX Control v3 Illumina Catalogue FC-110-3001). ..



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